Black Seed Oil Weed

Natural alternatives to CBD There are numerous concerns about the growing use of CBD oil in the medical industry, but not many healthcare providers are aware of the large number of natural Cannabis-induced Moto-Cognitive Dysfunction in Wistar Rats: Ameliorative Efficacy of Nigella Sativa Correspondence: Mr Aminu Imam, Msc (University of Ilorin, Nigeria), Department of Anatomy,

Natural alternatives to CBD

There are numerous concerns about the growing use of CBD oil in the medical industry, but not many healthcare providers are aware of the large number of natural products that have effects very similar to CBD.

CBD oil is currently being hailed as a new breakthrough for the treatment of epilepsy, tumours, anxiety, nausea, inflammation, spasms, insomnia and even psychoses or addictions. However, the presence of THC or tetrahydrocannabinol, the habit forming (addictive) psychoactive component of CBD oil as sourced from cannabis sativa or hemp is highly controversial. What works for one patient may be disastrous for another, depending on how well CBD suits their condition.

Alternative sources of CBD, such as nigella sativa, have no adverse effects on perception, sensory awareness, behaviour or reaction time. They are more affordable, easier to access and include a number of unique phytochemicals that are of more benefit than the cannabis species.

Some patients do not respond well to the effects of THC on CB1 receptors in the brain, however minimal the content may be. The “full spectrum CBD entourage” effect makes them feel high or groggy. Despite all the research and the therapeutic benefits that are coming to the fore some doctors are wary of prescribing CBD oil until they know more about it. CBD can interact with other medications as they are metabolized in the liver by the same enzymes as many other drugs via the CYP450 pathway. If you take antidepressants especially SSRIs (including Zoloft and Prozac) and opioids, seek medical advice before embarking on CBD.

Cannabidiol (CBD oil) is used for treating neural disorders such as anxiety, inflammation, migraines, pain and seizures. When made in the human body, these chemicals are called endocannabinoids but people with chronic diseases have a limited output, often due to micronutrient deficiencies, toxins, microbes or allergies to gluten, dairy and so on that are present. Are there any other sources of CBD’s that are free of such risks? There most certainly are!

The endocannabinoid system responds to CBD’s and has been modifying signals around the human body since Adam, using endocannabinoids (neurotransmitters) that we make ourselves. We have dozens of them to activate nerves and mediate pain, pleasure, anxiety, inflammation and other responses. Because these neurochemicals were first found in cannabis plants in 1940 they were dubbed cannabinoids. Since then other plants that contain similar chemicals were dubbed phytocannabinoids.

Some of them mimic the biological activity of the classic cannabinoid but have a slightly different structure and are called cannabimimetic compounds. Familiar herbal remedies, such as Echinacea, flaxseeds and nigella sativa (blackseed or kalonjie), for instance. These whole plant remedies provide a synergistic effect. As sources of CBD they have no adverse effects on perception, sensory awareness, behaviour or reaction time. They are more affordable, easier to access and include a number of unique phytochemicals that are of more benefit than the cannabis species. They are also free of THC, so do not have to grow in protected nurseries and do not require extensive testing and monitoring.

Nigella sativa (blackseed or kalonjie) as alternative to cannabis or CBD oil

The spicy, pungent little black seeds can be chewed or ground up and added to other spices or used as oil. Benefits include immune stimulation, anti-inflammatory and antioxidant effects, anticancer, hypoglycaemic, antihypertensive, anti-asthmatic, antimicrobial, cancer fighting and anti-parasitic activity. Apart from helping to control pain, epilepsy, Parkinsonism and anxiety kalonjie improves learning and memory, alertness and is mood elevating. In experimental animal models it was shown to prevent damage to brain cells from various neurotoxins.

1. Type 2 diabetes

Researchers found that just two grams daily of black seed could result in reduced fasting blood sugar levels, along with decreased insulin resistance and increased beta-cell function in the pancreas.

2. Epilepsy

A study in 1980 on 12 children with seizures showed that CBD (from cannabis) had antioxidant and neuroprotective properties. This led to a further study in 1988, funded by the National Institute of Health that showed that CBD treatment led to neurogenesis. At least 16 different studies support the use of black seeds (nigella sativa) as a natural anti-epileptic therapy, each revealing unique elements as to its clinical potential in remediating the onset, frequency, and duration of seizure episodes. According to the 2004 Journal of Phytomedicine, thymoquinone is a key constituent in the plant’s seeds with powerful anticonvulsant properties. As a result, patients experience shorter and less severe seizures plus nigella sativa protects against seizure-induced death (

3. Cancer

A growing number of studies show that thymoquinone from black seeds is an antioxidant and induces apoptosis of cancer cells. It is effective against cancer in blood system, lung, kidney, liver, prostate, breast, mouth, cervix and of the skin and colon. Thymoquinone extract reduced breast cancer tumor growth and increased apoptosis (cell death) in breast cancer cells. A study published in the online journal PLoS One indicates thymoquinone from black seed can induce cell death in glioblastoma cells, being one of the most aggressive of all brain tumors. It has also been shown to induce apoptosis in leukemia cells.

4. Bacterial infections – MRSA

The deadly and antibiotic-resistant bacterial infection known commonly as MRSA responded favourably to treatment with black seed in this study from the University of Health Sciences in Lahore, Pakistan.

5. Protection Against Heart Attack Damage

An extract from black seed has been shown to possess heart-protective qualities, and to limit damage associated with heart attacks and boosting overall heart health.

6. Brain Damage from Lead

A study published in Experimental and Toxicologic Pathology indicates black seed is able to protect the brain and reverse damage to the brain caused by lead toxicity.

Other plants with effects similar to CBD:


Recently it was discovered that flax (linen) seeds produce cannabinoid-like compounds that also very similar to CBD. They appear to have very similar anti-inflammatory effects, showing that flax products can be a source of biologically active cannabinoid-like compounds that are able to influence the cell’s immunological response ( There are not many studies that pursue this potential, but flaxseed oil is a well-known anti-inflammatory oil. It forms the major component of the Budwig protocol that is used as a very successful cancer treatment.


Commonly used as a cold and flu remedy and immune booster, echinacea’s CBD is similar to that of cannabis. However, echinacea modifies the CB1 receptor with cannabimimetics rather than cannabinoids. It’s this engagement with the endocannabinoid system that causes echinacea to relieve anxiety, migraines, fatigue, and arthritis. The N-alkyl amides (NAAs) contained in echinacea are very similar to THC in the way they regulate pain, inflammation, and the immune system.

Cannabis-induced Moto-Cognitive Dysfunction in Wistar Rats: Ameliorative Efficacy of Nigella Sativa

Correspondence: Mr Aminu Imam, Msc (University of Ilorin, Nigeria), Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria, Tel: +2348165663947, Fax: -, E-mail: [email protected]



Cannabis is a widely used illicit drug with various threats of personality syndrome, and Nigella sativa has been widely implicated as having therapeutic efficacy in many neurological diseases. The present study investigates the ameliorative efficacy of Nigella sativa oil (NSO) on cannabis-induced moto-cognitive defects.


Scopolamine (1 mg/kg i.p.) was given to induce dementia as a standard base line for cannabis (20 mg/kg)-induced cognitive impairment, followed by an oral administration of NSO (1 ml/kg) for 14 consecutive days. The Morris water maze (MWM) paradigm was used to assess the memory index, the elevated plus maze was used for anxiety-like behaviour, and the open field test was used for locomotor activities; thereafter, the rats were sacrificed and their brains were removed for histopathologic studies.


Cannabis-like Scopolamine caused memory impairment, delayed latency in the MWM, and anxiety-like behaviour, coupled with alterations in the cerebello-hippocampal neurons. The post-treatment of rats with NSO mitigated cannabis-induced cognitive dysfunction as with scopolamine and impaired anxiety-like behaviour by increasing open arm entry, line crossing, and histological changes.


The observed ameliorative effects of NSO make it a promising agent against moto-cognitive dysfunction and cerebelo-hippocampal alterations induced by cannabis.


Cannabis is a hemp plant in the genu flowering plants with three common species, including sativa, indica, and ruderalis (1). Preparations from cannabis, with common names like marijuana, weed, and grass, are the most consumed illicit drugs worldwide.

Cannabis dependency in users outnumbered that of other commonly abused drugs such as cocaine (2), and its exposure has been reported to enhance vulnerability to addiction and psychiatric disorders (3). The vulnerabilities include increased risk of other illicit drug use (4, 5, 6), enhanced intake and sensitivity to opiates (7), and enhanced cocaine-induced motor behaviour (8).

A wide range of neuropsychological studies have linked the consumption of cannabis to learning and memory impairments, as well as impairments to some aspects of executive functioning in humans (9, 10, 11), rodents (12, 13), and non-human primates (14).

It has been reported to impair mood, attention, short-term memory (STM), motor skills (15), and working memory (17), as well as to induce severe transient psychotic symptoms, lasting deficits in attention (16), and depressive disorders.

The major psychoactive constituent of cannabis, Tetrahydrocannabinol (THC), acts on the cannabinoid (CB) receptors that are widely expressed in the cerebral cortex, cerebellum, basal ganglia, Cornu Ammonis (CA)3 region of the hippocampus, and the dentate gyrus (DG) (18), resulting in wide topographical effects and functional alterations to the brain’s integrity.

Herbal and natural plant extracts have gained wide attention for their use in the treatment or management of neurological, psychiatric, and degenerative diseases based on their few or lack of side effects (19). Nigella sativa oil (NSO) is a high-value medicinal solvent used traditionally in the treatment of many diseases. Compelling evidence has shown that NSO exhibits protective activities against many diseases because of its high antioxidant (20) and anti-inflammatory properties (21).

A number of studies have been carried out, and the acclaimed medicinal properties emphasised in terms of the different therapeutic efficacies of NSO include neuro-protective (22), anti-asthmatic (23), anti-inflammatory, immunomodulatory, and anti-tumor properties (21), gastric ulcer healing (24), tumor growth suppression (25), male infertility improvement (26), and milk production stimulation (27). The main active ingredients in NSO are thymoquinone (TQ), alkaloids (nigellidine, nigellimine, and nigellicine), vitamins, such as thiamine, riboflavin, pyridoxine, niacin, and folic acid, minerals, and proteins (28).

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This study investigated the effects of cannabis sativa leaf extract on cognitive, locomotor, and anxiety-like behaviours, as well as on differential cortico-hippocampal histology in Wistar rats, and it evaluated the ameliorative efficacies of black seed oil on cannabis-induced moto-cognitive phenotypes and cortico-hippocampal histology.

Materials and Methods

Preparation of cannabis sativa extract

Cannabis plant leaves were obtained from the National Drug and Law Enforcement Agency (NDLEA) in Kwara State, Nigeria, and they were dried, blended to powdery particles, and weighed. The particles were dissolved in distilled water and kept for about 18 hours. The mixture was filtered, the filtrate was oven dried at 45°C, the dried filtrate was weighed, and then it was stored in an airtight container. Experimental approval was obtained from the NDLEA in Kwara State, Nigeria.


The Scopolamine hydrobromide used in this study was obtained from SIGMA, USA, and it was dissolved in saline to make a final concentration of 1 mg/; it was injected into the animals intraperitoneally (i.p.). The black seed oil (100% pure natural oil) was obtained from Masra warda, Kingdom of Saudi Arabia, and administer at 1 ml/

Animal care

Eighteen adult albino Wistar rats with an average weight of 200±20 g at the time of acquisition and acclimatisation were used in this study. The animals were housed in the animal holding of the Faculty of Basic Medical Sciences, University of Ilorin, Nigeria at six in a cage with free access to water and food and under a standard laboratory condition of 22±2°C temperature and a 12 h:12 h light-dark cycle.

Treatments schedule

The rats were randomly distributed into five groups (n = 6) as follows:

All procedures were scheduled and carried out during the light phase between 0900 and 1500 hours. All groups contain six rats each and treatments were given for seven consecutive days.

Morris water maze (MWM) test

The Morris water maze (MWM) is a common paradigm used for testing spatial learning and memory in rodents, and a modification of the procedure was used in the present study. The water maze used in this study consisted of a circular pool with a 1.6 m diameter and 50 cm height and water filled to a 44 cm depth, and it was coloured with a black non-toxic dye and had a controlled temperature of 25°C ± 1°C. Four equally spaced points around the edge of the pool were designated as north (N), east (E), south (S), and west (W). A black-coloured round platform with a 9 cm diameter was placed 1 cm below the water surface. The rats were trained to navigate the submerged platform. The rats were given a maximum time of 120 s (cut–off time) to find the hidden platform and were allowed to stay on it for 30 s. The platform remained in the same position during the training days. Rats that failed to locate the platform within 120 s were placed on the platform only in the first session. The animals were given a daily session of five trials. The escape latency time to reach the platform was recorded in each trial. Three subsequent tests were conducted, 24 h after the last training session on day six of this study. The escape latencies from the first two tests (I and II) were recorded, averaged, and then recorded as the long-term memory (LTM), while the escape latency recorded in the third test was considered the STM, respectively (29).

Open field test (OFT)

To assess the effects of the black seed oil on exploratory activity, experimental animals were evaluated in the open field paradigm. The paradigm is made of Perspex plastic with dimensions 40 × 60 × 50 cm, and the floor was divided into 25 equal squares by lines. Animals were individually placed in the centre of the apparatus and the numbers of squares crossed with all paws (frequent line crossing) were counted in a 5-min session, and all animals were monitored in a balanced design during the procedures (29, 30).

Elevated plus maze (EPM) test

To assess the anxiolytic activity of black seed oil (1 ml/kg orally) in rats, the elevated plus maze (EPM) paradigm was used. The EPM was made of two open arms (OA; 16 × 5 cm) and two closed arms (CA; 16 × 5–12 cm), and it was elevated (60 cm) above the floor. Rats were individually placed at the centre of the EPM with heads facing the OA, i.e., fear-inducing environment, they were allowed a 5-min test session, and the number of entries into the OA was recorded.


The rats were anaesthetised 24 h after the behavioural tests, decapitated, and their brains were excised and stored in a 4% paraformaldehyde preparation. Then, the brains were processed for histo-architectural examination, embedded in paraffin, refrigerated, coronally sectioned into 8-μm sections of the hippocampal formation (from Bregma −2.5 mm to −4.5 mm) and the cerebellar cortices (from Bregma −10 mm to −15 mm) using a rotary microtome (MK 1110), and stained with Hematoxylin and Eosin (H&E) for general cyto-architecture and Cresyl fast violet (CFV) for Nissl granulation acording to routine laboratory procedures (31). An Olympus BX 51 microscope and a DP 12 digital camera were used to produce photomicrographs from all the sections.

Statistical analysis

Data recorded in this study were reported as mean ± standard error of mean. The MWM, EPM, and OFT tests’ data were analysed using one-way analysis of variance (ANOVA) and for post-hoc analyses, we used the Bonfferoni test. A P-value of ≤0.05 was considered statistically significant in all cases. The software package Graph Pad Prism was used to analyse and graphically represent the data.


Cognitive functions

Cannabis significantly (P ≤ 0.05) impaired spatial memory tasks, causing a delayed latency to find the escape platform in the treated rat, an effect comparable to the deficit and delayed latency observed in the Scopolamine-treated rat. These effects are prominent in both LTM ( Figure 1 ) and STM tasks ( Figure 2 ) in the MWM.

Showing the delayed latency induced by scopolamine and cannabis, the unaffected latency in the control, an improved latency in the NSO and cannabis+NSO treated rats in LTM tasks in the MWM paradigm. Asterisks (*) indicate significant (P ≤ 0.05) lower escape latency of the carriers when compared with the scopolamine and cannabis treated rats.

Showing the delayed latency induced by scopolamine and cannabis, the unaffected latency in the control, an improved latency in the NSO and cannabis+NSO treated rats in STM tasks in the MWM paradigm. Asterisks (*) indicate significant (P ≤ 0.05) lower escape latency of the carriers when compared with the scopolamine and cannabis treated rats.

The potent efficacy of NSO in cannabis impaired working memory shows that administration of NSO significantly (P ≤ 0.05) reduced the latency to find the platform ( Figures 1 and ​ and2). 2 ). Therefore, rats treated with NSO after cannabis for seven days ameliorated the memory impairments caused by cannabis. Hence, the oral administration of 1 ml/kg/b.wt of NSO can reverse (P ≤ 0.05) the memory impairment induced by cannabis treatment.

Activities and anxiety-like behaviours

Locomotor activities and anxiety-like behaviours were assessed in the animals following cannabis exposure in the OFT and EPM paradigms, respectively. Cannabis markedly caused a significant (P ≤ 0.05) reduction in frequent line crossing in the OFT paradigm, while NSO only increased line crossing, but NSO after cannabis did not reverse the cannabis-induced deficit ( Figure 4 ). A complementary reduction in the open arm entry (OAE) following cannabis exposure was also recorded in the EPM, while NSO only and NSO after cannabis caused an increase in OAE ( Figure 3 ).

Showing reduced OAE following cannabis treatment, while NSO only and cannabis+NSO improved OAE in the EPM paradigm. Asterisks (*) and hash (#) indicate significant (P ≤ 0.05) lower open arms visit in cannabis treated animals and an improved open arm entry in the NSO only and NSO after Cannabis treated animals respectively.

Showing reduced frequent line crossing following cannabis treatment and cannabis+NSO, while high line crossing frequency were recorded in the NSO and control in the OFT paradigm. Asterisks (*) and hash (#) indicate significant (P ≤ 0.05) higher line crossing in NSO only treated animals when compared with Cannabis and Cannabis + NSO treated animals and a lower line crossing in the Cannabis and Cannabis+NSO treated animals when compared with the Control and NSO treated animals respectively.

Histopathological results

Sections of the cerebella cortices of the animals treated with amine, specifically the pyramidal cells at the internal pyramidal cell layer, show some degree of retraction of processes, vacuolation of the surrounding neuropil of the pyramidal cells, and hyperchromatic and shrunken perikarya ( Figure 7 ). Also observed are corkscrew-shaped apical dendrites and a blending of the membrane into the perikaryal cytoplasm ( Figure 7 ). NSO was observed in this study to enhance axonal-dendritic connections and to reduce scopolamine-induced vacuolation to nearly normal when compared with the control ( Figure 7 ). Scopolamine also caused irregular distributions of Nissl granulation in the perikaryal, increased dark pyknotic nuclei, and selective staining of Nissl granules (tigroid bodies and Nissl bodies), as well as the presence of a dark neuron, glial activation, and a dendritic cytoplasm, suggesting protein denaturing and neural degeneration in the hippocampus and cerebellar cortices ( Figures 8 , ​ ,9, 9 , ​ ,10). 10 ). NSO reversed these pathologies to almost normal when compared with the control.

Representative photo micrograph of the sections of the cerebellar cortices of rats following saline, cannabis, NSO and cannabis+NSO administrations showing: normal and unaffected cytology of the Pukinje cell with projection in A and C; marked affection of the Pukinje cells showing marked retraction of processes with vacuolations, and extreme shrinkening and blending in the perikarya cytoplasm in B, while D shows reduced vacuolation and distinct regenerations in the processes. (H&E ×400)

Representative photo micrograph of the sections of the hippocampi CA1 following saline, cannabis, NSO and cannabis+NSO administrations showing: normal nissl granulation in the mall pyramidal neuron (CA1) in A and C; pyknotic and darkly stained cell body, presence of dark neurons and vacuolation in the surrounding neuropil in B; reduced pyknoticity and disappearance of dark neurons in D. (CFV ×400)

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Representative photo micrograph of the sections of the hippocampi CA3 following saline, cannabis, NSO and cannabis+NSO administrations showing: normal nissl granulation in the large pyramidal neurons (CA3) in A and C; darkly stained cell body, vacuolation in the surrounding neuropil and shrunken perikarya in B; marked regeneration, less shrunken cells and contarasting soma stain in D. (CFV ×400)

Representative photo micrograph of the sections of the cerebellar cortices following saline, cannabis, NSO and cannabis+NSO administrations showing: normal nissl distributions in the soma and unaffected proximal dendrites of the large cerebellar Pukinje neurons in A and C; contrasting stains, pyknotic and darkly stained cell body, presence of dark neurons and vacuolation in the surrounding neuropil of Pukinje cells in B; marked regeneration, less shrunken cells. (CFV × 400)

The hippocampus is made up of CA1 and CA2 formed in a zone of small pyramidal cells, as well as CA3 and CA4 formed in a zone of large pyramidal cells. CA4 projects into the DG, made of small granule cells. The neuronal processes (axons and dendrites), glial cells, and scattered nerve cells are found in areas between the compact zones of the cells. NSO was able to repair the damage induced by Scopolamine exposure, including disorganization and a loss of small pyramidal cells (CA1), some of which had pale nuclei, while others were dark ( Figure 5 ). There was also marked shrinkage in the size of the large pyramidal cells (CA3), and the outer layer was more affected, with darkened nuclei and clumped processes ( Figure 6 ). Some darkened, pyknotic nuclei are observed in the granular cells of the DG with contrasting basophilia of some other granular cells’ nuclei; also observed are marked vacuolation and excess glial cells, effects ameliorated by NSO ( Figures 5 , ​ ,6, 6 , ​ ,7 7 ).

Representative photo micrograph of the sections of the hippocampi CA1 following saline, cannabis, NSO and cannabis + NSO administrations showing: normal and unaffected cytology of the small pyramidal neurons with well outlined soma and unaffected projections in A and C; vacuolation in the surrounding neuropil of the pyramidal cells, shrunken perikarya and blended membrane into the perikaryal cytoplasm in B and marked regeneration, reduced vacuolation and neuropil spaces in D. (H&E ×400)

Representative photo micrograph of the sections of the hippocampi CA3 following saline, cannabis, NSO and cannabis+NSO administrations showing: normal and unaffected cytology of the large pyramidal neurons (CA3) with outlined soma and unaffected projections in A and C; shrunken perikarya, blended membrane into the perikaryal cytoplasm and glia activation in B and D, but D shows reduced vacuolation and distinct regenerations in the processes. (H&E ×400)


Cannabis exposure can potentially function as a cumulative causal factor in some cases of schizophrenia, and its consumption by schizophrenic patients may likely pose a worsening outcome (32), but it remains one of most used illicit substances across boundless ethnicities, languages, classes, and regional diversities (33, 2), and it has been associated with management difficulties. Despite these facts, there is a growing perception that cannabis is safe and possesses medicinal potentials (34, 35).

The present study attempted to elucidate the motor-cognitive responses in cannabis administration in rats, the possible pathology in the hippocampus and cerebellar cortices, and the possible ameliorative efficacy of NSO following these alterations. The delayed escape latency recorded in the measures of LTM and STM in the MWM paradigm induced by cannabis is suggestive evidence of disrupted memory retention in the exposed animals, an effect similarly seen following exposure to Scopolamine, which is a standard amnesic model. Such effects of cannabis on the memory index, especially delayed latency in the MWM, have been previously reported in the literature (36, 37), thereby validating the present results.

NSO, which was hypothesised in this study as having the capacity to restore any memory dysfunction induced by cannabis, quickened latency to find the hidden platform in the treated animals when administered alone. Post-treatment with NSO after cannabis treatment also shows that NSO was able to ameliorate the cognitive dysfunctions induced by cannabis by reducing latency periods in both LTM and STM tasks in the MWM paradigm. These effects of NSO can be supported by various workers who also reported NSO reduced escape latency and increased target quadrant exploration in a MWM paradigm (29, 38), as well as reversed cognitive and novel object recognition in dementia modelled rats (39). Strengthened by various reports of its possible modulating impact on memory, attention, and cognition (40,41), a memory improvement amnesia model (29, 42) and reversed memory impairment in penthylenetetrazole (PTZ)-induced repeated seizures in rats (43) further confirm the memory enhancing and ameliorative capacity of NSO against cannabis-induced memory dysfunction, as observed in the present study.

The hippocampal pathologies observed in this study following cannabis administration in the CA1 and CA3 pyramidal neurons and the Nissl granulation can be supported by studies reporting distinct hippocampal morphological alterations in rats and humans following cannabis toxicity, including the presence of dark neurons, decreased sizes of nuclei, cellular infiltration, reductions in pyramidal cell density (44), reduced hippocampal CA1 and CA3 volumes, reduced DG volume (5, 45, 46), and reduced hippocampal gray matter densities in humans (47).

NSO administration in this study was observed to rescue the impaired hippocampal neural architecture following cannabis toxicity in the small and large pyramidal cells of the CA1 and CA3 regions. These effects could be strengthened by evidences from the literature reporting an improved neuronal cell viability, protection against beta-amyloid protein intoxication (48, 49), protection against cerebral ischemia reperfusion injury in the hippocampus (50), hippocampal neurodegeneration (51), and the prevention of hippocampal neuron cell death in global cerebral ischemia (20). Also supporting the results of this study is evidence of the efficacy of NSO in neurodegenerative diseases, including Parkinson’s and Alzheimer’s, due to its antioxidant potential (51, 52, 53), a property linked to the presence of TQ, which has the potential to prevent neuronal cell death (54).

The effects of cannabis on anxiety-related behaviors (OAEs), an indicator of disrupted emotional behaviours and line crossing frequencies, a marker of activities observed in the EPM and OFT paradigms, show more consistency regarding the anxiogenic properties. As observed in this study, the deleterious effects of cannabis on OAE in the EPM are demonstrated by accumulating evidence showing that cannabis causes a stronger dislike for the open arms of the EPM (55), thereby increasing anxiety-like behaviours (56, 57, 58). Nonetheless, this effect was not reversed by NSO in this study.

The reduced effects of cannabis on activity behaviour in this study are in countenance with earlier studies, where a depression in locomotor activities was reported following cannabis exposure (36, 59). These activity-related deficits and cytological alterations observed in the Pukinje cells of the cerebellar cortices may be linked to the affinity of cannabis to the CB receptors that is highly expressed in the cerebral cortex, cerebellum, and basal ganglia. These effects are repaired to an appreciable amount by NSO, a property that can be associated with its anti-inflammatory efficacy, as it has been reported to ameliorate cerebellar inflammation and demyelination in experimental autoimmune encephalomyelitis-induced rats (60).


In this study, cannabis impaired delayed escape latency in the MWM, thereby impairing memory and reducing OAE and frequent line crossing in the EPM and OFT, inducing anxiety-like behaviour and impaired activities. Post-treatment with NSO ameliorated the delayed latency and anxiogenic effects of cannabis. It also improved cortico-hippocampal granulation and the histo-architecture. Hence, NSO could be a potential supplement in the management of memory dysfunction and emotional deregulation seen in cannabis psychosis and toxicity.


We appreciate the Faculty of Basic Medical Sciences, University of Ilorin for the faculty research support and provision of routine histological consumables.


Ethical Approval

All experimental procedures were performed in accordance with institutional guidelines for animal care and use, and ethical approvals were obtained from the University of Ilorin Ethics Committee and the NDLEA in Kwara State, Nigeria.

Conflict of Interests


The authors received no financial support from any government or not for profit organizations to conduct this work.

Authors’ Contributions

Conception and design: IA, AMS, AMI, BWG

Analysis and interpretation of the data: IA, AA, AWI, IA, OOJ, AOA

Drafting of the article: IA, AA

Critical revision of the article for important intellectual content: IA, AMS, AA

Final approval of the article: IA, AMS, AA

Provision of study materials or patients: AMS, IA, AA, AA

Statistical expertise: AWI, IA

Administrative, technical, or logistic support: IA, AMS

Collection and assembly of data: AA


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