Gamma radiation effects on seed germination, growth and pigment content, and ESR study of induced free radicals in maize (Zea mays)
The effects of gamma radiation are investigated by studying plant germination, growth and development, and biochemical characteristics of maize. Maize dry seeds are exposed to a gamma source at doses ranging from 0.1 to 1 kGy. Our results show that the germination potential, expressed through the final germination percentage and the germination index, as well as the physiological parameters of maize seedlings (root and shoot lengths) decreased by increasing the irradiation dose. Moreover, plants derived from seeds exposed at higher doses (≤0.5 kGy) did not survive more than 10 days. Biochemical differences based on photosynthetic pigment (chlorophyll a, chlorophyll b, carotenoids) content revealed an inversely proportional relationship to doses of exposure. Furthermore, the concentration of chlorophyll a was higher than chlorophyll b in both irradiated and non-irradiated seedlings. Electron spin resonance spectroscopy used to evaluate the amount of free radicals induced by gamma ray treatment demonstrates that the relative concentration of radiation-induced free radicals depends linearly on the absorbed doses.
Keywords: Gamma ray, Maize hybrid, Germination potential, Growth parameters, Photosynthetic pigments , Electron spin resonance spectroscopy
Research on the basic interaction of ionizing radiation with biological systems has contributed to human society through applications in medicine, agriculture, pharmaceutical uses, and other technological processes. Gamma rays have proved to be more economical and effective compared to other ionizing radiations because of their easy availability and power of penetration .
The exposure of a biological system to ionizing radiation activates a number of physical and chemical steps between the initial absorption of energy and the final biological injury. One of the most important targets is the water molecule, which is omnipresent in organisms. The ionized water molecule (H2O and the radicals H and OH are produced due to the primary reactions of excitation and ionization. In biological tissues, these ionizations are induced all along the radiation path, leading to chain reactions that produce secondary free radicals as a result of H and e becoming trapped . These radicals can damage or modify important components of plant cells and affect certain physiological and biochemical processes that might be vital for organism survival. Previous studies [3, 4] have revealed that seed exposure to high doses of gamma rays disturbs the protein synthesis, hormone balance, leaf gas exchange, and enzyme activity. The morphological, structural, and functional changes depend on the strength and duration of gamma doses of exposure.
The measurements of free radicals in food and biological systems by electron spin resonance spectroscopy (ESR) are widely used in the fields of food irradiation, lipid oxidation, antioxidants, and food processing [5, 6]. Free radicals are chemical compounds that possess an unpaired electron in the outer shell of a molecule and due to this unpaired electron, these species are paramagnetic .
Zea mays is one of the world’s most important crop plants, boasting a multibillion dollar annual revenue. In addition to its agronomic importance, maize has been a keystone model organism for basic research for nearly a century. Within the cereals, which include other plant model species such as rice (Oryza sativa), sorghum (Sorghum bicolor), wheat (Triticum spp.) and barley (Hordeum vulgare), maize is the most thoroughly researched system. As a model organism, maize is the subject of far-ranging biological investigations such as plant domestication, genome evolution, developmental physiology, epigenetics, pest resistance, heterosis, quantitative inheritance, and comparative genomics .
Considering the effects of radiation on plants, the present study was conducted to determine the effects of radiation on maize growth and development and on the content of photosynthetic pigments. Furthermore, we used ESR spectroscopy to study the behavior of a radiation-induced free radical in gamma-irradiated maize seeds and to correlate it with the germination pattern.
Materials and methods
Seeds of maize hybrid Turda Star were obtained from the Agricultural Research and Developmental Station, Turda, Romania, and were submitted to gamma treatment.
Irradiation of maize seeds was performed using a 60 Co (Cobalt 60) gamma source (Gamma Chamber 900) in ambient conditions at the Physics Faculty, “Babeş-Bolyai” University. The doses of exposure were 0.1, 0.2, 0.3, 0.5, 0.7, and 1.0 kGy. The control maize seeds were not irradiated.
Radiation sensitivity test
Three replicates of 20 seeds each/treatment were sowed on moistened filter paper and then germinated at 20 °C with an 8 h photophase, in laboratory conditions. Seeds were considered germinated when they exhibited a radical extension of >2 mm. Counts of germinated seeds were made daily for 7 days to determine both the final germination percentage and germination index. The final germination percentage (FGP) was calculated according to  as follows:
where NT = the proportion of germinated seeds in each treatment for the final measurement; N = number of seeds used in the bioassay.
The germination index is a quantitative expression of germination that relates the daily germination rate to the maximum germination value. The germination index (GI) was calculated as described by the Association of Official Seed Analysts  by the following formula:
where N1, N2, N3 and Nn represent the number of seeds that germinated in day 1, 2, 3. n
The root and shoot lengths of control and irradiated samples were measured on the seventh day after the start of the experiment. Shooting and rooting were defined as the protrusion of the shoot and root to the extent of at least 0.5 mm. The root/shoot length data represent the mean of the three replicated treatments.
Pigment extraction was carried out essentially as described by . Twenty-eight-day fresh maize leaves were cut into small pieces (2–3 mm), which were suspended in DMF (N,N– dimethylformamide), approximately 100 mg vegetal material/2 ml DMF. The samples were incubated for 48 h in the dark at 4 °C. Absorbance measurements were read in a spectrophotometer (ultraviolet- visible [UV/VIS] spectrophotometer SP8001 Metertech Spectrophotometer provided by the Department of Experimental Biology and Biochemistry, Institute of Biological Research, Cluj-Napoca, Romania) at 664-, 647-, and 480-nm wavelengths. Using the absorbance mean values chlorophyll a, chlorophyll b, and carotenoid pigments were calculated based on equations proposed by  and expressed in μg/g fresh weight [FW]. The data represent the averages of the three replicated treatments.
Gamma-irradiated maize seed samples were placed in ESR quartz tubes in order to register paramagnetic species. ESR spectra were recorded at room temperature with the Bruker Biospin EMX spectrometer operating at X-band (9–10 GHz). The ESR parameters were set at 100-KHz modulation frequency, microwave power of 2 mW, modulation amplitude of 2 G, time constant of 2.56 ms; scan time of 61 s, number of scans, five, and receiver gain 10 4 . The variation of the steady state of the relative concentration of the paramagnetic species generated at different absorbed doses was obtained through double integration of the experimental spectra divided by the sample weight expressed in milligrams.
Experimental data were subjected to one-way analysis of variance (one-way ANOVA) with Dunnett’s post test, at a 5% level of probability, to determine the differences in average of all tested parameters between irradiated and non-irradiated plantlets. Statistical analysis was performed using GraphPad Prism (version 5.00 for Windows, GraphPad Software, San Diego, CA, USA).
Results and discussion
Radiation sensitivity test
The seed germination test after gamma irradiation (0.1–1.0 kGy) revealed that the maximum germination percentage was observed in control seedlings. As illustrated in Fig. Fig.1, 1 , the final germination percentage decreased with increasing gamma ray doses by 11% at 0.1 kGy, 22% at 0.2 kGy, 34% at 0.3 kGy, 40% at 0.5 kGy and 45% at 0.7 kGy. The maximum decrease, by 62%, of the germination percentage was observed at 1 kGy. Statistical analysis revealed that exposure at doses higher than 0.1 kGy significantly decreased the seeds’ germination capacity at 7 days from gamma radiation exposure.
Effect of gamma irradiation on final germination percentage and germination index of maize (Each bar represents Mean ±SD [Standard deviation] for average of n = 3 independent experiments; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001)
Seed germination potential, expressed through the germination index (Fig. (Fig.1), 1 ), revealed the same decreasing pattern by increasing the radiation doses, like FGP. Exposure to a dose of 0.1 kGy decreased insignificantly the germination index by 11% but a statistically significant inhibition of the germination potential was noticed at higher doses, where the germination index decreased by 21% for 0.2 kGy, 28% for 0.3 kGy, 32% for 0.5 kGy and 41% for 0.7 kGy, comparing with the control. The highest inhibition of the germination process, by 51%, was recorded at 1 kGy.
The biometric measurements of roots emerged from irradiated seeds show a significant decrease of length after 7 days from the start of the experiment (Table (Table1). 1 ). The maximum radical length was recorded in the control samples, while the radical length of samples exposed to 0.1, 0.2, 0.3, 0.5 and 0.7 kGy decreased by 9, 31, 41, 47, and 56%, respectively. The maximum reduction of radical length, by 71%, was observed at 1 kGy. Results show that gamma treatment with doses higher than 0.1 kGy significantly inhibited the length of the radicular system of plants derived from irradiated seeds.
Effects of gamma radiation on root and shoot mean length (cm) of maize (Zea mays)
|Gamma irradiation dose (Gy)||Root length (cm)||Shoot length (cm)|
|1.85 ± 0.23||0.81 ± 0.01|
|100 Gy||1.67 ± 0.18||0.73 ± 0.01|
|200 Gy||1.26 ± 0.07 ∗||0.49 ± 0.08 ∗∗|
|300 Gy||1.08 ± 0.18 ∗∗||0.42 ± 0.14 ∗∗|
|500 Gy||0.97 ± 0.14 ∗∗||0.38 ± 0.01 ∗∗|
|700 Gy||0.81 ± 0.04 ∗∗∗||0.32 ± 0.001 ∗∗∗|
|1000 Gy||0.52 ± 0.03 ∗∗∗||0.30 ± 0.03 ∗∗∗|
Data are the means of three replicates ± SD [Standard Deviation]; ∗ Significant difference (p < 0.05); ∗∗ Highly significant difference (p < 0.01); ∗∗∗ Extremely significant difference (p < 0.001)
The gamma rays (≥0.2 kGy) imposed a significant impact on the shoot length. The highest shoot length was observed in the unirradiated plants. Following exposure to gamma rays, shoot lengths decreased by 11, 40, 48, 53, and 60% at 0.1, 0.2, 0.3, 0.5, and 0.7 kGy, respectively. The maximum decrease of shoot length, by 63%, was observed at 1 kGy (Table 1 ).
Plants derived from seeds exposed to higher doses (≥0.5 kGy) did not survive, so it was not possible for further investigations.
These results are in accordance with the findings of previous researchers who reported that the seed germination potential of different crops decreased by increasing the irradiation dose. Mashev et al.  showed that irradiation with doses above 0.1 kGy caused a significant decline in the grain yield of wheat. Gamma treatment (0.1, 0.2, 0.3, 0.4 kGy) of three wheat cultivars caused a delay of the germination process and decrease of the survival percentage and plant height .
Growth inhibition induced through high-dose irradiation has been attributed to cell cycle arrest in the G2/M phase during somatic cell division and/or to a variety of damages in the entire genome . Processes like auxin destruction, changes of the ascorbic acid content, and physiological and biochemical disturbances could induce the inhibition of plant germination and development . Chaudhuri  reported that when radiation is sufficient to reduce the rooting percentage, the root lengths do not exceed a few millimeters in length. Due to metabolic disorders in the seeds after gamma irradiation, the seeds are unable to germinate or to survive more than a few days. Plant survival to maturity depends on the nature and extent of chromosomal damage. The frequency of chromosomal damage with increasing dosage may be responsible for less germinability and reduction in plant survival and development .
Photosynthetic pigment content
The contents of chlorophyll a, chlorophyll b, and carotenoids were determined only for samples exposed to 0.1, 0.2, and 0.3 kGy, because the plants derived from seeds exposed at higher doses (≥0.5 kGy) did not survive long enough to allow leaf growth and development.
The contents of assimilatory pigments, both for the control and irradiated samples, are presented in Fig. Fig.2. 2 . Statistical analysis showed that the chlorophyll a content has significantly decreased in plant leaves derived from irradiated seeds. Comparing with the control, 92.92 μg/g FW, the chlorophyll a content decreased by 27% at 0.1 kGy (67.27 μg/g FW), 33% at 0.2 kGy (61.85 μg/g FW) and the maximum decrease, by 39%, was recorded at 0.3 kGy (56.38 μg/g FW).
Effect of gamma irradiation doses on chlorophyll a, chlorophyll b, carotenoids, total chlorophyll, and total assimilatory pigments content (μg/g FM) of maize (Each bar represents Mean ±SD for average n = 3 independent experiments; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001)
Regarding chlorophyll b, a similar trend of decreasing content with increasing gamma dosage as in chlorophyll a (Fig. (Fig.2) 2 ) is noticed. Chlorophyll b significantly decreased by 13% and 42% at 0.1 kGy (28.38 μg/g FW) and 0.2 kGy (18.79 μg/g FW), respectively. A remarkable decline of chlorophyll b was recorded with 0.3 kGy plantlets, which exhibited a content of 11.56 μg/g FW, which was 64% lower compared with non-irradiated samples, 32.63 μg/g FW.
Carotenoids were also negatively influenced by gamma treatment. Compared with the control (19.68 μg/g FW), gamma treatment with 0.1 kGy (11.56 μg/g FW) and 0.2 kGy (9.03 μg/g FW) induced a significant decrease of the carotenoids content by 41 and 54%, respectively. As seen in Fig. Fig.2, 2 , the minimum content of carotenoids is noticed in samples exposed to 0.3 Gy, 5.83 μg/g FW, which represents a decrease by 70% comparing with the control.
Our data are in agreement with the results of previous works [19, 20] where chlorophyll concentrations were found to be lower in irradiated plants than in control ones.
Photosynthetic pigments can be destroyed by gamma rays with a concomitant loss of photosynthetic capacity. Saha et al.  reported that the chlorophyll content of plants decreased gradually after irradiation, which can result from the release of chlorophyll from its protein complex with subsequent dephytolization and possibly pheophytinization. In addition, it can be observed that the concentration of chlorophyll a was relatively higher than the concentration of chlorophyll b in both irradiated and unirradiated seedlings. A number of researchers [18, 22] have already reported that gamma irradiation results in greater destruction of chlorophyll b than chlorophyll a due to disturbance of its biosynthesis or degradation of its precursors.
The ESR spectra of typical gamma-irradiated glass powder samples measured at room temperature are shown in Fig. Fig.3 3 and are characterized by a structureless signal, centered on a g-factor of 2.0073 and a line width of 8.1 G.
ESR spectra of gamma-irradiated maize at some absorbed doses
The nature of the paramagnetic species in the non-irradiated samples has been attributed to oxidation of fatty acids visible in some vegetables, phenols, especially flavonoids or as free radicals of semi-quinones or lignin .
Gamma irradiation was reported to induce oxidative stress with overproduction of reactive oxygen species such as superoxide radicals, hydroxyl radicals, and hydrogen peroxides, which react rapidly with almost all structural and functional organic molecules including proteins, lipids, and nucleic acids causing disturbance of cellular metabolism .
Reactive oxygen species can react with nearly all cell constituents. Such interaction triggers free radical chain reactions, eventually causing membrane lipid peroxidation. As a result, the membrane loses its stability and its permeability is enhanced leading to damages of the cell structure and disturbances of normal physiological functions . Massive doses of ionizing radiation have been shown to induce physiological changes in plants, such as enhancement of respiration, increase in ethylene production and induction of enzyme activities (particularly for phenolic metabolisms and accumulation specific protein species). These effects are considered a consequence of both the direct interactions between the ionizing radiation and the macromolecular structures and the indirect action of ROS generated by water radiolysis .
Polovka et al.  and Suhaj et al.  confirmed, using EPR spectroscopy, that the gamma radiation treatment of cellulose-containing spice samples such as ground black pepper (Piper nigrum L.), allspice berries (Pimenta officinalis L.), ginger root (Zingiber officinale Rosc.), dried clove buds (Caryophyllus aromaticus L.) and dried oregano leaves (Origanum vulgare L.) resulted in the dose-dependent generation of paramagnetic species of different structures and properties.
The double integrated ESR signal per 1 mg of samples was used to follow the variation of paramagnetic species as a function of absorbed dose (i.e., dose–response curve). A linear increase of the relative concentration of the paramagnetic species is observed (Fig. (Fig.4 4 ).
Variation of relative concentration of paramagnetic species as a function of the absorbed dose
In the present study, treatment of maize seeds with high doses of gamma rays ( 60 Co) showed the same decreasing pattern of all studied parameters (final germination percentage, germination index, root and shoot lengths, photosynthetic pigment content) with an increase of irradiation dose. Gamma radiation exposure at doses ranging from 0.1 to 1 kGy lead to a decrease by 11–62% of the final germination percentage, by 11–51% of the germination index, by 9–71% of the root length, and by 9–62% of the shoot length. Plants derived from seeds exposed to higher doses (0.5, 0.7, and 1 kGy) did not survive to allow further investigations. The photosynthetic pigment content was also decreased by 26–49% at 0.1, 0.2, and 0.3 kGy compared with the control.
Unirradiated and irradiated ground maize was studied using ESR spectroscopy. Gamma radiation induced detectable amounts of free radicals in the studied samples. The relative concentration of the radiation-induced free radicals was found to depend linearly on the absorbed doses. Results of the present study substantiate the suitability of employing the ESR technique for detection of free radicals present naturally or produced after radiation and/or conventional processing.
The present results show that seed treatment with 60 Co gamma radiation (0.1–1 kGy) decreased plant germination, development, and the photosynthetic pigment content with a concomitant increase of the relative concentration of the paramagnetic species. These findings confirm the results obtained by earlier studies that showed the inhibitory effects of plant growth and development exposed to high doses of gamma radiation, simultaneous with the increase of reactive oxygen species generated through water radiolysis.
The authors wish to express their gratitude to the Department of Experimental Biology and Biochemistry, Institute of Biological Research, Cluj-Napoca, Romania, for providing some infrastructure facilities to carry out the experiment and providing access to laboratory equipment.
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Your opinion: Gamma radiation for decontamination of medicinal cannabis
It is no news that gamma radiation is utilized by companies that produce medicinal cannabis flowers in countries with national medicinal cannabis programs, such as The Netherlands and Canada. A recent study, published by Arno Hazekamp, Head of Research and Education at Bedrocan International BV, has sparked a discussion again, because the procedure remains controversial.
Pharmaceutical regulations demand gamma radiation treatment of herbal cannabis in order to meet strict safety standards regarding the (possible) microbial contamination of the plant. A study named “Evaluating the Effects of Gamma-Irradiation for Decontamination of Medicinal Cannabis” has analysed four different strains of cannabis, produced by Bedrocan, before and after the radiation process, and has tested changes in terpenes, cannabinoids and moisture content.
While no changes in THC, CBD nor moisture content of the medicinal cannabis could be identified, the research showed damage to a few terpenes. “Some terpenes are reduced, but no new compounds are formed. This means terpenes are evaporated to some extent, but not degraded into new compounds. Gamma irradiation somehow accelerates the evaporation just a little bit, but does not kill or destroy your cannabis.”, Hazekamp suggests.
The study further concluded that gamma radiation remains the safest and most efficient technique to make sure patients, especially those that with compromised immune systems, receive a safe product. The original, full length research article, including discussion and conclusion, can be found here.
Patients’ organizations react to the procedure of gamma radiation
However, patients, consumers and activists remain sceptical and express concerns about gamma radiation.
According to the research of patients’ organizations, like Canadians for Safe Access, one of the side effects of gamma radiation is the production of a new type of chemicals, which don’t occur naturally. Cancer-producing chemicals can develop as well, when fat is exposed to the procedure.
Derrick Bergman (known from Encod and VOC) has published a paper with the clear title “Medicinal cannabis in the Netherlands: most patients prefer coffeeshop over pharmacy”. In the document he expresses that some patients claim gamma radiation has negative effects on the quality of medicinal cannabis, while Bedrocan states that the process is a demand of the Office of Medical Cannabis.
The Dutch patients’ group PGMC (Stichting Patiënten Groep Medicinale Cannabis Gebruikers) points out that said study is missing the answer to one very important question: What happens to the irradiated products when vaporized/smoked or eaten?
In this article, we have collected a range of different opinions on this subject for you. Please feel free to share yours with us and the Sensi Seeds audience.
What Is Medicinal Cannabis, and How to Choose a Medicinal Cannabis Strain?
Gamma radiation for decontamination of medicinal cannabis – a collection of different quotes
It’s also important to remember that gamma-irradiation is not just done without good reason. It is meant to prevent the potential greater harm of infecting a patient with harmful microbes. In my paper several sources are cited where this actually happened. It is weighing two ‘bad’ choices (risk of infection vs. irradiation) and picking the most sensible one.We should all hope for the development of new sterilization techniques for cannabis in the near future. – Hazekamp reasons on news.liftcannabis.ca [x]
…Gamma irradiation produces un-natural compounds (ionizing radiation causes a multitude of chemical changes)… – Reverend Ryan says on leafscience.com [x]
Why the obligatory Gamma radiation? And what is the effect? – says James Burton, Founder of the Dutch medicinal cannabis program [x]
You are constantly irradiated by the sun. If you don’t tan, there is no problem. Something that is irradiated to kill germs DOES NOT become radioactive – otherwise everyone who goes outside into the sun would be a health hazard when they came in – not to mention a mass of cancer! – D. J. Walters commented on cbc.ca [x]
I don’t think irradiation will dissuade customers. There will be some initial grumbling and outrage, but it will be eventually accepted as the norm. Most of us use dried spices when we cook and that’s all irradiated. I don’t think it will be the issue that kills the program. Wouldn’t a blind test be a fun thing to try on this issue? – leaffan commented on rollipup.org [x]
Does radiating cannabis with gamma rays have an effect on the compounds? No, it just sterilizes any seeds. – Steve Harris said on quora.com [x]
RADIATION. I have never known a home grower that needed to use this kind of poison. Just another reason NOT to get your medicine from these people. Are they going to tell the patients if it’s been radiated or not? – Bmunro commented on cbc.ca [x]
Gamma irradiation does not eradicate mould spores… This is perhaps the biggest misconception about this unholy and unsafe procedure and now also proven to be ineffective too. So patients in the NL don’t only suffer from ineffective cannabis .. but from ineffective gamma radiation perpetrated on an inferior product and then put in unsterile inadequate packaging too. This is a crime and torture! – Jackie Woerlee commented on marijuanapatients.org [x]
I wasn’t in favor of the gamma ray. – James Burton has a clear stance on the topic
What’s the point of irradiating medicine when the natural thing with organic supplement is better than what’s in our tobacco?? … – pfk 182 commented on rollipup.org [x]
Benefits of Organic Cannabis – A How-To Guide for Growing Your Own
I develop X-ray based irradiators for use in biological studies. The recent market development of medical marijuana has begun to look into treating marijuana as a standard agricultural product. I will not go into GMO vs Organic as I am not an expert on the subject. However; I can speak to say that irradiating organic material does not make the product radioactive. This is a common misconception among the general population which is simply not true. Irradiating organics is not the practice of sprinkling radioactive material onto the product. This does not happen. But it is using the energy release from a radioactive source in order to sterilize and kill the bacteria. Radiation is just invisible light with a different wavelength than what you get out of your flashlight. Your concerns are the same as fearing to eat something you pulled out of the microwave because the microwave radiation would poison you. Consider the sun, if you stand outside in the sun for a period of time your skin will burn. But if you go inside, away from the sun’s UV radiation, then you don’t continue to burn. Same concept. The alternatives to gamma/X-ray are using UV, which alters the chemical composition of the weed as well as the effects. Or chemical sterilization. – Robert commented on marijuanapatients.org [x]
Prairie Plant Systems uses a sterilization process called gamma irradiation to ensure its product is free of bacteria and mold. And that indeed involves exposing it to radiation. On the other hand, irradiation is a common and approved method for sterilization, especially in the pharmaceutical and agricultural industries where quality control is tightly regulated. …ensuring that medical marijuana is toxin-free is important for patients with immune system deficiencies. Bedrocan B.V., the only legal producer of medical marijuana in the Netherlands, also irradiates its product. – states an article on leafscience.com [x]
Wow, so gamma radiation does convert fats/acids/terpenes in cannabis, which then makes it carcinogenic. Great – says Drifter on weedforum.eu [x]
And this is one of the reasons I am returning to black market weed. It’s a higher quality, lower price, more reliable service and not radioactive… – Steve commented on marijuanapatients.org [x]
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Gamma radiation for decontamination of medicinal cannabis – your opinion please!
Do you agree or disagree with the comments above? What are your thoughts on gamma radiation for the decontamination of medicinal cannabis? Are you for, or against it? Let us know what your point of view is. You can do so by commenting on this post in the section below.
To a fair, respectful debate!
Laws and regulations regarding cannabis cultivation differ from country to country. Sensi Seeds therefore strongly advises you to check your local laws and regulations. Do not act in conflict with the law.